Immunocytochemical Pre-Screening of Stained Cell Smears



In recent years proteins within cells have been identified that are directly related to particular diseases, e.g. cancer. Many of these proteins can be visualized with immunocytochemical marker stains. For example cells infected by a virus can be visualized by attaching a stain to antibodies which in turn bind to a specific upregulated protein after virus infection. The antibody, docked to the affected cell, is stained by a chromogene which then can be identified in a microscopic inspection. Human papilloma viruses (HPV), e.g., are responsible for almost all cervical cancers, and the infection can be detected with the specific p16-antibody in cervical brush smears with high sensitivity.



A PAP smear, stained with a counter stain (hematoxylin, blue) and the p16-antibody (brown).



An immunocytological investigation can therefore non-invasively identify high risk patients for this type of cancer. The automated scanning of slides followed by computer aided detection of marker-positive cells will help physicians to cope with the high load of specimens in a screening scenario and to reach a more reliable diagnostic result.



A newly developed virtual destaining of immunocytological specimens reveals the seperation between the immunocytochemical marker stain (brown) and the counter stain (blue).




David Friedrich, André Bell, Kraisorn Chaisaowong, Till Braunschweig, Ruth Knüchel-Clarke and Til Aach
High Dynamic Range Microscopy for Color Selective Virtual De-Staining of Immunocytological Specimens
In: Bildverarbeitung für die Medizin 2011


André Bell, David Friedrich, Kraisorn Chaisaowong, Till Braunschweig, Ruth Knüchel-Clarke and Til Aach
Fast Virtual Destaining of Immunocytological Specimens
In: IEEE International Symposium on Biomedical Imaging (ISBI) 2011


Research topics

Ahmet Aydin
Improving Specificity of Immunocytochemical Marker Detection via 40x Magnified Microscopic Images.

Tobias Wächter
Detection of stained vaginal mucus to reduce artifacts in immunocytochemical pre-screening.
(Detektion von eingefärbtem Vaginalschleim für die Artefakt-Reduktion im immunzytochemischen Pre-Screening)

Joschka zur Jacobsmühlen
Development of detection algorithms for immunocytochemical markers in microscopy images.
(Entwicklung von Detektionsverfahren für immunzytochemische Marker in Mikroskopie-Bildern)


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